No heats detected for the first couple of injections for ITC?

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Please advise theoretical aspect of business management in construction ?

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When using the relative quantification method in the 7500 PCR system, is a reference sample essential?

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Assay cascades - how do they work in DMTA cyle?

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Marco Signorelli

it's normal; the first injection is lower than the other because there is diffusion during the dwell time. The solution is, for esample, selected first injection 1 microliter and the other 10 microliters.

Adrian Velazquez-Campoy

Before saying anything else, could you provide a figure from your experiment?

Sam Darby

Thank you for your help. It turns out it was simply not injecting the volumes. The problem is now sorted. Cheers!