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I am doing ligation of my gene size 2.7kb in vector pet28a at 160C overnight. After that I transform my mixture in DH10B or DH10alpha. Most of the time i get no colonies and sometimes i get very...
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Hello, I am trying to express a protein of 47kDa in E.coli. I have sequenced my gene in pet28a. The sequence is correct and i tried codon plus cells but my protein is not expressing. I have tried...
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