I did liver MNCs isolation for several times and I could not detect NKT cells by using NK1.1 & TCRb or α-GalCer-loaded CD1d tetramer&TCRb. During all these experiments, I used 40μm cell strainer, which was not commonly used in this isolation. Common protocol suggest 200-gauge steel mesh or 100μm nylon mesh. Is this the reason? But since lymphocytes size is less than 20μm, I do not think this is the problem. Does anyone know the reason?