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Questions related from Zhengzuo Li
Hi All, I just got a weird image from Beckman Coulter Cytoflex S. So I stain Liver mononuclear cells with APC-NK1.1 and BV605-TCRβ, then I got the following image. The left lower quadrant shows...
05 May 2018 1,145 3 View
I recently got a problem with my secondary Ab, it is PE goat anti-rat IgG and it stained B6 mouse liver lymphocytes non-specifically. But when I incubated primary Ab and secondary Ab for 3 hours...
05 May 2018 4,135 3 View
I stain NKT cells in the spleen by using NK1.1 and TCRβ. As was shown in the figure, I did detected NKT, but it was not very clear. I increased Ab concentration or PMT voltage, but it did not...
05 May 2018 9,351 4 View
I did liver MNCs isolation for several times and I could not detect NKT cells by using NK1.1 & TCRb or α-GalCer-loaded CD1d tetramer&TCRb. During all these experiments, I used 40μm cell...
03 March 2018 9,524 2 View
I need to establish a ConA induced liver injury model now and I want to buy ConA from Sigma. But I found there are so many types of ConA and I did not know how to choose. Does anyone know? Here is...
03 March 2018 2,230 1 View
Anti-mTIM-1 Ab(Clone:3B3) was used in flow cytometry to detect TIM-1 expression on T cells. When I use the same Ab to repeat this experiment, I always got the negative results. I think Staining...
02 February 2018 551 6 View
I am dealing with 3-color panel design now, they are: PE, APC and BV605. If I am gonna use FMO control in experiment, do I still need to do compensation before experiment?
02 February 2018 7,279 4 View
