A colorimetric method for measurement of carbamylated haemoglobin in patients with chronic kidney disease using spectrophotometry. J medicine and med sciences 2012, vol 3(8).
We are working on the same aspect as mentioned in the article above, but are facing certain technical issues. When ninhydrin was dissolved with butanol and acetic acid, a double layer of liquid was formed. So we used ethanol instead of butanol along with acetic acid to dissolve ninhydrin. There was no layer formation, but the readings were not in agreement with the high and low standard respectively.
In continuation of the above question, any suggestions to solve the issue with using butanol as solvent for ninhydrin?
Hi Suganthi,
A mixture of butanol , acetic acid and double layer formation you can over come the problem by adding water to the mixture in small portions and mixing or using other V:V mixture ratio.Good luck
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