Nile Red staining protocol?

07 February 2025 1 2K Report

I have few specimens fixed in 100% ethanol. Can I use Nile Red for CSLM or is it just possible in animals fixed in PFA?

Thiago Q. Araujo , To be honest: I would just like [edit: to] point you to 4 protocols / descriptions I've in my e- collection. I don't know whether either of those protocols fits your need (CLSM). Since I am not able to attach these articles / papers in this RG-post / form (due to Copyright-restrictions) I'd encourage you to send me an RG-message and I gladly will forward those articles to you as "collegial" kindness / favour. best regards, W. H. M.

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