Dear scientists. Have any of you published the results of changes in gene expression obtained by next-generation sequencing? The analysis was carried out in endothelial cells of cows with some gene silenced in comparison to cells with the gene not silenced. We expected the expression changes to cover a maximum of several dozen genes, however, the results show a difference in expression for over a thousand genes. There is so much data that we wonder what to publish and what to omit. Could you advise on what to focus on when presenting such results in the publication?
Hi Robert
as for CRISPR you have your targeted genes but there are maybe some off-targets genes, explaining the rise in the number. there is also an other explanation, the impact of your targeted genes in the expression of their biological targets...
all the best
fred
just see the stats on the differently expressed genes and put your list of genes in database as stringDB (https://string-db.org) to target the pathway you discovered in your paper.
changing one gene can have an impact on so many other genes. but don't forget that cultured cells are very sensitive to all environmental factors as phone use (Article Mobile phone radiation causes changes in gene and protein ex...
).
all the best
fred
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