I have optained new derivatives of MPA. And now I am thinking what is the best way to check their biological activity (without rats etc) on lymphoid cell line and human peripheral blood mononuclear cells? Ealier I made MTT test, proliferation test and IMPDH inhibition test. Now I would like to change a bit of methodology. Can I use CellTrace violet instead of tritium labeled thymidine? Could I also replace the MTT test with something? Could I also use flow cytometry with 7-AAD? What is the best way to check IMPDH inhibition? Maybe you can suggest ma also another test which could be useful?

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