Do anyone can give me advice of how to derived cardiac cells from spheroid I already try to produce neurosphere and is was quiet easy.
phil
Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...
01 July 2024 5,102 0 View
Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...
29 April 2024 6,779 1 View
plastic revolution?
10 April 2024 4,191 2 View
Hi everyone, everything is in the title question ! To the best of your knowledge, what is the shortest sequence you know that translate into a fluorescent protein that could be used for flow...
07 March 2024 1,762 1 View
Hi everyone, I'm currently working on the HaCaT keratinocyte cell line. I have them in culture under low and high calcium concentration to keep them undifferenciated or differenciated,...
04 March 2024 2,153 0 View
Hello everyone, I use several pcDNA3.1 expression vectors to transfect cells. The vectors were prepared by midi-prep a year ago and diluted in TE buffer. Now that I run new experiments, I...
04 March 2024 1,867 3 View
I work on a MODFLOW Model using GMS to simulate subsurface water exchanges between a small straightened stream and its heavily modified alluvial plain. I’m looking for efficient methods to modify...
09 January 2024 708 0 View
Hello everyone, I'm curently working on HaCaT cells which require low calcium medium to return to undifferentiate state. To do so, I need to chelate my FBS for 1h with chelex resin among other...
12 December 2023 4,975 2 View
In an action-research, our research team is conducting interviews to identify key moments about professional practices in the field of education with participants.
04 December 2023 2,114 1 View
Hello, I was not able to find a natural inhibitor for Fibroblast Activation Protein (FAP) in the banks or in the literature. Even among broad spectrum inhibitors Is there any identified? Thank...
26 November 2023 636 2 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?
29 July 2024 7,714 4 View
In some data sources it has been grouped in Q1 and some shows it is Q2.
29 July 2024 4,227 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View