How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Can someone help me with tissue histology of mice?

I've been trying to assess the efficacy of a drug that I've purified in mouse model. I've induced mammary tumors in balb/c mice and treated them with my drug. I've harvested the tumors, and other...

16 December 2020 3,515 3 View

Can anyone help to get this research paper on OCB?

Turnipseed, D & Murkison, G (2000). Good Soldiers and Their Syndrome: Organizational Citizenship Behavior and the Work Environment, North American Journal of Psychology 2(2)

11 November 2020 7,488 3 View

What are some landmark texts and papers on agroecology we need to follow for a clear understanding?

We often read a lot about sustainable agriculture or conservation agriculture (or recently, climate-smart agriculture) but it is often difficult (especially for early-stage researchers) to fathom...

08 April 2020 9,212 10 View

Can anyone share me a better image of the attached map? In which book the map was originally published?

The map is reproduced in Eaton, Richard M. The Rise of Islam and the Bengal Frontier, 1204-1760. Berkeley: University of California Press 1993; reference given are: Barrie M. Morrison,...

13 August 2019 1,485 2 View

Does 4T-1 cells induce ascites in mice?

I am new to in vivo research. I have tried to induce solid tumors in mice by injecting 4T-1 cells in the mammary fat pad, which is an established procedure. While i mostly ot solid tumors, 2 or 3...

18 June 2019 973 3 View

How to solve problems arising from choosebank() command in R?

I am using seqinr package in R. But when I am giving the command choosebank() to find out the existing databases or commands like choosebank('swissport") to open any specific database, R is...

15 April 2019 1,541 3 View

What is the scope of Fortran in machine learning?

I intend to use ML for molecular dynamics simulations and want to know of packages available in Fortran. Would it be more efficient to use some other language instead for the long run?

09 January 2019 8,295 2 View

Which regression/multivariate technique to apply if dependent var is continuous scale, cant be categorised, non-normal, Indep Variables dichotomous?

02 September 2018 1,981 2 View

For droplet-based sc-RNA sequecing data, library size varies across cells. How does one tackle this when calculating statistical variance for genes ?

To calculate the statistical quantities like variance or mean expression of a gene across cells, it is unfair to compare expression counts of a say gene A, if we know different cells has not be...

14 July 2018 10,016 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View