Need to know if higher IPTG concentrations make E. coli expression host undergo a metabolic stress?

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Atoshi Banerjee

yes IPTG halts the divison process but enhances protein production. however, if we increase IPTG beyond a limit the divison of bacteria is compromised and which in turn effect the protein machinery of the cells.

Ditsa Sarkar

Yes, a high concentration of IPTG is toxic to the cell. Usually, in order to induce a cell, we do not go beyond 1mM of IPTG.