Why would you think that chemokine receptors can differentiate T cell subsets? I have never seen studies to suggest that they can.

Cytokines are difficult, so most use nuclear factors, such as tbet, GAT3, etc... While the nuclear factors are not absolute, they should be good enough for what you need.

Dear Shen-An Hwang

T Helper cell subsets do differentially express chemokine receptors, right?

If that's true then why can't we use them as surface markers?

Thanks in advance.

That's what I'm saying, T cell helper subsets do not differentially express chemokine receptors.

The only time I've seen differentially expression of chemokine receptors is between effector, memory, effector memory, etc, and these type of T cells. And even then, most still use other markers in addition to chemokine receptors for identification.