Need help with Bionumerics. ?

More Ben Hassena Amal's questions See All

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Polymer wear calculation?

What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?

04 August 2024 1,078 1 View

Why is it not common to see similar pairs in polymer sliding pairs?

If the pairs are similar, will it reduce the adhesion due to electric charges?

29 July 2024 3,185 2 View

Hot to do MTT assay data analysis?

I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...

08 July 2024 1,900 1 View

How to amplify NCF1 with real time PCR without amplifying its pseudogenes NCF1B, NCF1C ?

I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !

04 July 2024 3,068 3 View

I need content writers, poets, researchers to my new online magazine, would you be interested?

Good day! I am the CEO of an online magazine called Order Of The Pen . We specialize in promoting new writers via our platform on Wix and Partnership with Mailchimp for email marketing,...

18 June 2024 4,117 0 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

How to plot in Simu5G on Omnet++?

Dear network I need to plot instantaneous and mean End-to-end delay in D2D and eNode-based topologies? How to generate/visualise the accurate vec file data? I appreciate your help

14 May 2024 2,897 1 View

What is the best control for conditioned medium?

I'm planning a small research project involving conditioned medium from cancer cells on a human cell line (specifically KGN cells). I intend to expose the cancer cells to this medium for 72 hours...

06 May 2024 8,470 2 View

Can someone advise me on how to do the simulation of polymer-to-polymer sliding, especially for thin films?

I am trying to understand the influence of polymer films sliding against each other. For the simulation, I saw many molecular dynamics simulations, like LAMMPS, GROMACS, etc. I am new to this...

09 April 2024 8,871 2 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

PFGE why there are no or few bands?

Hello, I am new in PFGE and started by checking Lambda ladder electrophoresis accordingly to BIO-Rad recomendations: -CHEF DR III -gel in 0.5x TBE, -recirculated at 14 °C. -Run time was 22...

18 March 2024 2,830 4 View

Confusion about virus DNA PFGE image ?

This dot appeard instead a visible distinct band. the concentration of the DNA was 30 microgeam/ml Can you help me please if this is a band or not and why it appears like that ?

10 January 2024 4,187 3 View

How can I unde the accidentally deletion of entries in Bionumerics ? Is there an undo function ?

How can I unde the accidentally deletion of entries in bionumerics ? Is there an undo function ?

20 July 2023 9,770 0 View

Does any one have any information regarding Pulse field gel electrophoresis facility available in India especially on southern parts?

Any information regarding the availability of PFGE facility in Tamilnadu?

13 June 2023 7,859 2 View

How to resolve DNA digestion problem using PFGE ?

Hi everyone, I hope someone can help me to resolve my problem. I am trying to perform PFGE on archaea (Thermococcales) but I do not understand why my genomic DNA gives a smear and not visible...

29 December 2022 2,791 6 View

Help!! How to make the PFGE of Bacillus cereus success?

I did the PFGE of Bacillus cereus many times, but there were no bands on the gel. The protocol reference is https://pubmed.ncbi.nlm.nih.gov/25862049/ What's the problem with the experiment?

04 November 2022 7,122 0 View

Can anyone suugest a good alternative to GelcomparII or Bionumerics Software?

Hello, I intend to analyze my DGGE gels and need a good software to do the same. Specifically, I want to check the band densities and construct dendograms. Can anyone suggest any good software...

08 February 2022 9,996 3 View

PFGE on Burkholderia thaliandensis?

Hi all, I am new to PFGE and I was wondering if anyone has done them specifically on B. thailandensis. I am having trouble with the ramped pulsed times. Additionally, I am not getting any bands...

11 January 2022 5,884 4 View

Does anyone have a favorite PFGE gel plug preparation method for isolating HMW DNA, and also the best agarose to use?

We are trying to isolate intact HMW DNA for our large scale genome projects. Thanks in advance.

10 November 2021 6,983 1 View