Need CRISPR/cas9 2015-16 full text articles, or possible downloadable links, will be truly grateful
We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...
22 February 2021 2,072 3 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance
09 February 2021 717 6 View
I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...
08 February 2021 2,793 2 View
I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...
08 February 2021 2,032 2 View
I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...
02 February 2021 8,083 1 View
Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...
31 January 2021 2,516 1 View
I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.
26 January 2021 8,733 2 View
Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...
14 January 2021 1,170 2 View
For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...
11 January 2021 6,016 3 View