Hello everyone, I am currently conducting immunofluorescence (IF) staining of tyrosine hydroxylase (TH) in brown adipose tissue (BAT) and white adipose tissue (WAT). I am using paraffin-embedded samples and the Anti-tyrosine hydroxylase antibody (Pel Freez Biologicals P40101-150). However, I have encountered difficulties obtaining a positive signal.

I would greatly appreciate it if anyone could provide a detailed and effective protocol for the IF staining of TH in adipose tissue. Your expertise and guidance would be immensely valuable to my research.