Does anyone have a protocol for an NADPH oxidase activity assay to use on tissue?
We will be looking at hippocampal tissue, if tissue type matters.
Good day! I am currently testing my sample for MIC, may you help me how to interpret the results at OD630? how would I know if there is growth or not based on the values given by the microplate...
01 June 2024 7,920 3 View
My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.
27 May 2024 8,228 2 View
Need advice on user-friendly tools to ease meta-analysis process of selecting eligible and fugitive studies
02 May 2024 2,332 3 View
Hi, I am currently constructing a phylogenetic tree involving heterokonts, specifically of marine diatoms and dinoflagellates. Looking at the BLASTn results of my query sequence, I am wondering...
30 April 2024 4,239 2 View
The γ-tubulin Ab i got gives me a 48kDa band in hepa cell samples but when i try it in mouse liver tissue samples i get a weird band at 48 and another one at almost 70kDa. Ive tried a different Ab...
23 April 2024 9,475 0 View
Someone has requested a copy of an article I co-authored but I don't see options to respond. When I clicked on "View Request," it took me to a page with information about the article but not...
19 April 2024 6,368 1 View
Can the reasons for such an incident be detailed?
13 February 2024 2,795 4 View
Kindly research on how AI is going to impact on legal sevices and in particular arbitraration.Right now some firms have started using AI for research,due diligence and data analytics.
12 February 2024 4,005 5 View
Our goal is to synthesize graphene oxide (GO) utilizing biomass graphite through a modified Hummers method. However, we get rGO straight away. How is rGO produced in the absence of a reducing...
01 February 2024 7,346 3 View
There is no available sca here in the PH, and we need to isolate Actinomycetes. Thank you in advance!
20 January 2024 4,713 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View