I have performed NADH oxidase and succinate dehydrogenase assay from mitochondrial fraction from the cestode parasite Hymenolepis diminuta which undergoes anaerobic energy metabilism. I want to know the process of calculating the specific activity of the enzymes and also the calculation for percentage inhibition after treatment with plant extracts.
Enzymatic Specific Activity (AS) corresponds to micromoles of products appeared per minute and per mg of protein. It is also expressed in the form: U / mg.
It can be calculated from the Enzymatic Activity (micromoles of producr formed per minute and per mL of protein, ie U / mL) determined experimentally and the concentration of protein in solution (mg / mL):
AS = AE / C
Thank you very much for your kind response. Actually, my question would have been "how to calculate the enzyme activities". As I shall know the enzyme activities, I shall be able to calculate the specific activity as well. It was my mistake to ask a wrong question. So, I rectify it to "how to calculate the activity of NADH oxidase and Succinate dehydrogenase in an anaerobically energy metabolising organism?"
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