Hello Daniel MacKeigan ,

Is the SPR chip modified/functionalised in any way? Does the integrin that you are using have any anchroing group available?

The following article used amine coupling, I think they cite a previous paper for a more comprehensive overview of their immobilisation method:

Article Integrins v 3 and 4 1 Act as Coreceptors for Fractalkine, an...

I also attach an article (Brown et al., 2015) outlining in detail the formation of a non-fouling mixed self-assembled monolayer on the gold-coated SPR chip with a controlled density of reactive carboxylic acid groups; They then activate this surface using EDC/NHS (200 mM and 50 mM, respectively, at a flow rate of 2 µL.min-1 for 10 min. Finally the integrins were coupled to the activated surface with a density of 100 µg.mL-1 and flow rate of 5 µL.min-1 for 10 min.

Hope it helps,

Jules

Hi Jules, that information is very helpful thank you so much! I'm new to SPR so I'm unsure whether it is functionalized at all, only that it is gold plated. And that is definitely more integrin than I thought might be required as the chip is 2cm x 2cm. But I will check out those papers and test whether doing this is possible in my lab.

All the best,

Daniel

When you are new to SPR, the www.sprpages.nl are a great resource!

Kind regards

Arnoud