How to deal with the recudtion of amplification efficiency of indexed primers?

Hi all, I am now working with ITS2 sequencing library preparation. I had observed that after introducing adapters, linkers and barcodes to a primer, the indexed primers have a much lower...

27 January 2021 5,045 3 View

Are there any DNA intercalators that do not have fluorescence?

I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?

25 December 2020 688 3 View

How to interpret the centrality indices in network analysis?

I learned that in order to interpret the centrality indices, the CS coefficients resulted from a bootnet test are preferably above 0.5 and above 0.25 is acceptable. In my network, the CS...

29 October 2020 3,545 3 View

How to create a graph of psychopathology symptoms when conducting network analysis in R?

I've been working on the network analysis of adolescent depressive symptoms. I've estimated the network, the centrality indices and network stability using packages qgraph and bootnet. Then I want...

22 October 2020 5,591 3 View

How to interpret the results of NetworkComparison Test when conducting a network analysis in R?

25 September 2020 9,936 2 View

Does anybody know how to use the Lagranto model?

These days I wanted to run the Lagranto model to track water vapor transport path. But I got some trouble in installing an available version of Lagranto model. I attempted to use "conda install -c...

04 July 2020 5,417 4 View

Abaqus & Fortran complier linking problem ?

Hi, I am trying to run a vumat subroutine, but there is some abaqus-fortran linking issue. I use abaqus 6146 Micrasoft visual studio 14.0 parallel studio XE 2016 I added these command in my .bat...

25 February 2020 5,698 2 View

Health informatics: Relationships between industry development and academic research?

In digital health domain, is the academic research that leads the development of the industry OR is the industry development that impacts the associated academic research ?

10 June 2019 5,214 6 View

Fluorescence Polarization Assays?

When I titrated fluorescence probe by Protein A alone or by Protein B alone, it do not should any binding signals, mP do not change. However, when I titrated the same fluorescence probe by...

18 March 2019 2,468 3 View

How does esiRNA from Sigma compare to Dharmacon's SMARTpool siRNA?

I would like to know information about esiRNA form Sigma for the following points: 1. transfection protocol; 2. knockdown efficiency; 3. off target effects; 4. toxicity. Anyone would like to share...

31 May 2018 9,661 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What test should I use?

15 February 2021 911 5 View

What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View

In Method prepreation, what is the meaning of (Fixed at), and the meaning of (mol% of somthing) and how to find the volume?

I need an explanation of this sentence: Firstly, SnCl4.5H2O with fixed at 0, 1, 3, and 5 mol% of TiO2 and Titanium (IV) isoproxide (TTIP) with fixed at 10 ml were mixed into 150 ml of ethanol...

10 February 2021 3,291 2 View