My UV only gives 3.00 as ABs value at 280 nm for solutions of different concentrations and even for a cuvette with nothing inside. How to fix it?

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Has anyone used MALDI-TOF to analyze DNA molecules （MW > 20000）?

I am seeking advice on the matrix and solvent conditions.

29 May 2024 2,360 0 View

Dear， How to define sexual spore?

I found it very difficult to define it precisely. Microspore and megaspore of ferns or seed plants are sexual spore? Because the fertilization happened for a long time before the formation of...

27 April 2024 3,077 0 View

How to effeciently connect triethoxysilane to glass?

Hi all, I'm Dabin Xie from Zhincai Tech, a small research-driving company in China. I'm currently trying to modify glass with a single layer of silane whether hydrophobic or hydrophilic, like...

08 April 2024 2,628 4 View

Temperature difference is insufficient to perform the heat transfer ?

How to solve this problem when Aspen economic evaluation was performed? It said "Temperature difference is insufficient to perform the heat transfer."

06 April 2024 7,291 3 View

Which software is better for simulating superconducting magnetic fields?

If I want numerical simulation software to simulate superconducting magnetic shielding, which software is better for simulating superconducting magnetic fields?

25 March 2024 5,589 1 View

Unanticipated Self-Recombination of pENTR Observed Post LR Clonase Reaction?

I've encountered an issue following an LR reaction intended to construct a gene within a pENTR-3C (KanR) vector and combine it with a pCDH (AmpR) vector. Despite expectations, the protein was not...

14 February 2024 3,502 0 View

In PyMOL, Encoder "ffmpeg", "mpeg_encode", and "convert" are not installed. How to install these encoders?

I tried to make a moive of protein structure in PyMOL. However, when I stored the movie for future use, the PyMOL reminded that the encoders were not installed. How to install these encoders in...

29 October 2023 5,129 2 View

How to perform the 3C -qPCR analysis when I do not have BAC clone as control?

I have performed the 3C-qPCR assay and designed the primers of interested region, but I do not have BAC clone as control. How should I normalize the prime efficiency and background noise?

16 October 2023 4,387 0 View

Are TiN(111) and TiN(222) different crystal planes in XRD pattern?

In the XRD experimental analysis of polycrystalline TiN films, are TiN (111) and TiN (222) different crystal planes? Or are they the result of different diffraction levels of the same crystal...

26 September 2023 3,580 5 View

Can PyMOL show a closed cyclic protein (N-and C-terminus closed after reaction)?

If a protein was designed as a closed cyclic protein (N-and C-terminus closed after reaction), can PyMOL show this structure? Because in PyMOL, the sequence is always linear, so how the closed...

17 September 2023 2,886 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

I am trying to find phonon, and when i run calculations, there is error ?

how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.

23 July 2024 4,649 2 View

How to fix install package meta in R?

> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’

18 July 2024 3,536 0 View

GIS (How can i fix when it appear in mines despite selecting WGSWGS 84 / UTM zone 38N?

I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?

18 July 2024 2,696 1 View

Why in some wavelengths there are "overflows" in my Carbon dot UV-Vis Spectroscopy results(Emission segment)?

Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...

18 July 2024 4,244 2 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

How can I put small FWHM in CasaXPS?

I'm trying to fitting Cu Auger peak into various state (Cu0, Cu+, CuI etc..) In Cu0, one of the peak's FWHM is 0.84 but when I try to enter smaller value than 0.89, "input error" is occured. How...

02 July 2024 4,932 3 View

Any Green Solvents to dissolve PET near room temperature?

I need a PET + Solvent mixture to feed it to a fixed bed reactor for steam reforming.

30 June 2024 2,122 2 View