My question is about calsium alginate!?

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Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

What can be the mechanism for this reaction in attached file?

Cross coupling for insertion of the chromophore

20 February 2024 9,433 0 View

How do you calculate a subgroup's mean and SD when you have the mean and SD of all other subgroups and the whole mean and SD?

Hi everyone! Recently, I've been working on a systematic review and meta-analysis, and I needed to calculate EDSS (a disease severity score) for multiple sclerosis patients. Imagine that a study...

19 February 2023 7,700 2 View

Is there any maximum number for studies that can be included in scoping review?

Is there any maximum number for studies that can be included in scoping review? This is Homa Arshadi, Ph.D. Candidate of medical library and information sciences. I need some help with the...

29 November 2022 6,037 4 View

How to analyze the complexity of double deep Q-learning (DDQN) algorithm?

How could I analyze the complexity of the double-deep Q-learning (DDQN) algorithm? Is it sensible to compare it with a normal deep neural network? Is the required time for convergence with a...

03 April 2022 4,502 4 View

Can you help me find a conference in field of urban disaster recovery?

Dear Researchers. I am looking forward to finding a well-known or prominent conference that has proceedings in disaster recovery, urban resiliency, sustainable development, and community...

01 November 2020 8,864 2 View

How cell wall peroperties of l.lactis may change after surface display of a heterogenous antigen?

Hi there! We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm...

03 August 2019 7,558 2 View

How to explain positive significant coefficient turning negative nonsignificant on adding another variable in OLS regression?

I am using Stata 13.1 to estimate the potential trade of Iran (gravity model). Before that, I have to get the coefficients of the model. The problem is, the exporter economic size coefficient gets...

31 July 2018 6,641 9 View

How can I determine the intact bacterial cell in flow cytometry?

Hi guys! I'm working on a whole cell SELEX project of bacteria for developing a diagnostic tool. Most of bacteria are less than 2 micrometer in size that is interfered with many noises . Please...

11 March 2018 8,308 5 View

Why FITC-nucleotide conjugate emission vanish during SELELX process?

Hi there! I'm using FITC-primer conjugate for amplification of DNA library. The florescent tag is necessary for investigation of SELEX proceeding by flow cytometry technique. The problem is...

03 February 2018 5,798 8 View

Is skin yellowness an numerical or ordinal variable?

I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...

11 August 2024 4,793 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Why applying a saved template of a bar chart in a different/new one doesn't take effect in SPSS?

Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...

29 July 2024 1,981 1 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View