How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

What are the impacts of pKa1and pKa2 in drug absorption ?

Pregabalin is a crystalline solid with a pKa1 of 4.2 and a pKa2 of 10.6. what insight can i get from this aforementioned information during drug design. What is the difference between pKa1and pKa2.

28 December 2020 7,237 3 View

How to deal with negative factor loadings while performing PCA-MLR to calculate quantitative source contribution in air pollution studies ?

If I am getting some negative values in factor loadings while performing principal component analysis (PCA), then I should use that negative values to calculate daily source contribution using...

22 December 2020 5,248 1 View

What are the source markers of Naphthalene, Acenaphthylene and Acenaphthene in ambient atmosphere ?

Is any article available which reports the sources of these PAHs ?

22 December 2020 8,738 1 View

Why high infiltration in OM treated plot?

We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...

25 November 2020 8,787 3 View

Where can one download data (csv, excel) for average daily values of AQI (air quality index) in popular cities, like, Delhi and Mumbai in India?

Air pollution has become a very big problem in Indian cities, especially Delhi. It requires immediate attention and we need to soon understand the trends, provide forecasts for future, issue...

19 November 2020 1,433 3 View

What is the best feasible method to plot DISTANCE vs LST graph?

The above question is intended to be answered in reference to the reduction of the urban heat island by the river.

22 October 2020 7,732 4 View

Which type of measuring instrument can be used to measure hot thermic fluid flow rate?

The flow rate of thermic fluid is less than 1 L/min and temperature ranges from 25 - 200 degree Celsius with a pressure limit of 5 bar.

06 October 2020 5,612 11 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

How to calculate a flow rate over the broad-crest wear for this conditions?

How to find a relationship between velocity and weir landing length (X') as shown in figure?

28 September 2020 7,159 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What test should I use?

15 February 2021 911 5 View

What kind of tape can be used to fix the samples on an XRD machine?

I have a very thin sample, the thickness is around 1 mm. I want to do X-ray diffraction measurement on the thin side of the sample. What kind of tape can I use to fix the sample, and will not...

13 February 2021 4,153 3 View

In Method prepreation, what is the meaning of (Fixed at), and the meaning of (mol% of somthing) and how to find the volume?

I need an explanation of this sentence: Firstly, SnCl4.5H2O with fixed at 0, 1, 3, and 5 mol% of TiO2 and Titanium (IV) isoproxide (TTIP) with fixed at 10 ml were mixed into 150 ml of ethanol...

10 February 2021 3,291 2 View