Dear community,
Trying to find a murine NK cell line that expresses Ly49 genes. Has anyone worked with any such cell line? I would highly appreciate it if you could kindly share your experience!
Regards
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
Hello everyone, I'm curious to know if anyone has experience using Silvaco for simulating 2D memory. I'm particularly interested in exploring the memory characteristics of two-dimensional...
14 May 2024 7,781 0 View
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10 May 2024 8,189 0 View
Hello! When constructing the plasmid, I used the same promoter. However, the direction of each expression cassette promoter in the obtained plasmid is the same, and the plasmid with the opposite...
07 April 2024 9,022 1 View
If I wanted to experimentally investigate the effect of information quality on rumour sharing intentions and the moderating role of information sharing motivation in this (we would manipulate...
24 March 2024 6,428 8 View
Is anyone interested in or looking for further collaboration on approximating the solution of nonlinear PDEs (of the diffusion-type) using the Laplace transform? In essence, its derived results...
19 March 2024 4,713 3 View
Hi everyone. Greetings to you all. Recently, I tested the viscosity of the epoxy resin and CF/epoxy prepreg samples using a TA-discoveryHR-1 rheometer. Firstly, in an isothermal way(holding...
19 December 2023 5,798 2 View
How to score and interpret single-item health status measures: a manual for users of the SF-8™ Health Survey
01 December 2023 2,371 0 View
There are two ways we can go about testing the moderating effect of a variable (assuming the moderating variable is a dummy variable). One is to add an interaction term to the regression equation,...
23 November 2023 4,265 16 View
Does anyone have a good resource for running fMRI power analyses? http://neuropowertools.org is down and fmripower seems to have been removed. Any ideas appreciated!
21 September 2023 922 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Air moisture harvesting Air water collection devices
06 August 2024 5,473 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View