How process to avoid cell detachment when removing the MTT before adding DMSO? because lot of blue formazan cristals detach (and even after a centrifugation of the plates) when using this type of cells.
I was doing the MTT assay on those cells, and it is true that they are very easily detaching, but you can manage to solve the problem. I am adding the 10x more concentrated solution of MTT into well, without removing the medium. Therefore you are skipping the one step of liquid removal. The second improvement - seed them on the 48 or 12 well plate. They like to grow in peculiar manner, resulting in empty spaces between 'colonies', so you will have more cells and they will be more attached. The third possibility is that you are using a high concentration of the drug, so they are either dying or arrested in mitosis (round and detached). Also you can try to dissolve the crystals in isopropanol, it is better and stable solvent.
I hope it would be useful.
Best luck,
Aga
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