Anybody has an idea whether mpk4 mpk6 double knock out mutants were ever studied?
I am looking for plasmid or bacteria expressing 6xHis-tagged cutinase from Erysiphe graminis for purification. Thanks a lot in advance
09 October 2017 8,348 0 View
Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...
10 November 2015 2,033 7 View
I need fluorophore conjugated anti-rat and anti-mouse immunoglobulins that do not crossreact. Anyone with a reliable source of cross-adsorbed IgGs of this kind?
03 April 2015 5,260 4 View
I am looking for efficient kits for Co-IP using mouse and rabbit IgGs. Effectively co-IP output should be minimally contaminated with IgG heavy/light chains. Any idea?
08 September 2014 4,823 10 View
I need a link to an online tool where I can upload small peptide sequences or FASTA format proteins that do not necessarily have a PDB accession. Any idea?
06 July 2014 7,084 2 View
Homo- or heterobifunctional crosslinkers were sparsely reported in the literature as putative fixatives for immunolocalization microscopic studies. Does anybody have information on which...
03 April 2014 1,715 1 View
Does anyone have experience with high pressure fixation regimes for plant tissues with different vacuolation degrees. Primarily I would like to apply HPF to Arabidopsis roots, but I would greatly...
02 March 2013 4,842 1 View
Does anyone have any ideas about high refractive index mounting media for either fixed, immunolabeled samples or for living, GFP-fusion expressing cells, to avoid spherical aberrations with high...
01 February 2013 8,317 2 View
Can somebody attach a step by step and thorough protocol for aqueous two phase partitioning of Arabidopsis root plasma membranes? I am not experienced and I need an easy to follow approach.
09 October 2012 5,080 4 View
For example formaldehyde, glutaraldehyde or acrolein? I am particularly interested to understand whether soluble proteins may be largely relocated during chemical fixation.
08 September 2012 9,186 26 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...
28 July 2024 4,762 2 View
Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.
28 July 2024 6,501 0 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View