Anybody has an idea whether mpk4 mpk6 double knock out mutants were ever studied?
I am looking for plasmid or bacteria expressing 6xHis-tagged cutinase from Erysiphe graminis for purification. Thanks a lot in advance
09 October 2017 8,258 0 View
Anyone has experience with a truly permeable DNA stain that will not be adsorbed in plant cell walls? We work on Arabidopsis roots and we tested dyes that are membrane permeable in animal cells...
10 November 2015 1,906 7 View
I need fluorophore conjugated anti-rat and anti-mouse immunoglobulins that do not crossreact. Anyone with a reliable source of cross-adsorbed IgGs of this kind?
03 April 2015 5,160 4 View
I am looking for efficient kits for Co-IP using mouse and rabbit IgGs. Effectively co-IP output should be minimally contaminated with IgG heavy/light chains. Any idea?
08 September 2014 4,722 10 View
I need a link to an online tool where I can upload small peptide sequences or FASTA format proteins that do not necessarily have a PDB accession. Any idea?
06 July 2014 6,985 2 View
Homo- or heterobifunctional crosslinkers were sparsely reported in the literature as putative fixatives for immunolocalization microscopic studies. Does anybody have information on which...
03 April 2014 1,595 1 View
Does anyone have experience with high pressure fixation regimes for plant tissues with different vacuolation degrees. Primarily I would like to apply HPF to Arabidopsis roots, but I would greatly...
02 March 2013 4,737 1 View
Does anyone have any ideas about high refractive index mounting media for either fixed, immunolabeled samples or for living, GFP-fusion expressing cells, to avoid spherical aberrations with high...
01 February 2013 8,180 2 View
Can somebody attach a step by step and thorough protocol for aqueous two phase partitioning of Arabidopsis root plasma membranes? I am not experienced and I need an easy to follow approach.
09 October 2012 4,977 4 View
I want to ask whether growth conditions of Arabidopsis seedlings could affect the efficiency of transgene expression driven by the 35S promoter of cauliflower mosaic virus. I am having problems...
08 September 2012 9,864 26 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...
28 February 2021 9,138 2 View
What HPLC Marker(s) or standard is used to detect and quantify anthocyanins such as Delphinidin, Cyanidin in a given sample of plant extract?
23 February 2021 1,832 4 View