I have flash frozen some brain slices in OCT with isopentane, cooled by liquid nitrogen
I have mounted some on slides using a cyrostat however I am finding that the tissue dissociates from the slides very rapidly.
I just wondered if anyone had any advice? It was deemed unsuitable to fix the tissue with PFA before storing in the -80
Thanks
Hi Anya, so right now you have tissue that is not fixed, technically a fresh frozen tissue sample. At what point exactly is the tissue dissociating [I see you're using these samples for IHC most likely]?
I have flash frozen tissue the same way you did, however, when I sliced in OCT, they were always fresh and not flash frozen. I would then freeze the OCT cubes and section & mount from there.
I have used that method of flash freezing before with succes.
I used Superfrost Plus™ Adhesion Microscope Slides by Thermo Scientific. These have a special coating that makes the tissue stick to the slides better. Maybe you could try that.
Hi Anya Snary ,
I agree with Martin Schol , I use Superfrost Plus™ Slides by Thermo Scientific which electro-statically attract frozen tissue sections. I mount with them directly from sectioning in the cryostat and the tissue is adhered to it properly.
Good Luck!
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