Anya Snary

4 Questions 1 Answers 0 Followers

Questions related from Anya Snary

Why do my high MW markers on SDS-page disappear?

I make 10% SDS page gels and run at 110 v for 1.5 hours My marker separates initially however as it progresses the top MW markers disappear (the ones I need!) I have tried different % gels, new...

01 January 2021 8,290 3 View

Mounting snapfrozen tissue using a cyrostat - best preservation method?

I have flash frozen some brain slices in OCT with isopentane, cooled by liquid nitrogen I have mounted some on slides using a cyrostat however I am finding that the tissue dissociates from the...

08 January 2020 9,089 3 View

Is there a way to quantify protein concentration of FACS sorted cells?

Hey all, I've been trying to quantify the protein of FACS sorted cells via using colourimetric assays, however, have realised that due to the nature of the fluorophores this has impacted the...

27 September 2019 6,203 0 View

Why embed brains in agarose before organotypic slicing?

Hi all, I'm currently troubleshooting an organotypical culture procedure and I've seen a lot of methods embedding the fresh tissue in agarose then slicing with a vibratome - I just wondered how...

01 August 2019 5,846 3 View