Anya Snary

3 Questions 1 Answers 0 Followers

Questions related from Anya Snary

Why do my high MW markers on SDS-page disappear?

I make 10% SDS page gels and run at 110 v for 1.5 hours My marker separates initially however as it progresses the top MW markers disappear (the ones I need!) I have tried different % gels, new...

01 January 2021 8,150 3 View

Mounting snapfrozen tissue using a cyrostat - best preservation method?

I have flash frozen some brain slices in OCT with isopentane, cooled by liquid nitrogen I have mounted some on slides using a cyrostat however I am finding that the tissue dissociates from the...

08 January 2020 8,963 3 View

Why embed brains in agarose before organotypic slicing?

Hi all, I'm currently troubleshooting an organotypical culture procedure and I've seen a lot of methods embedding the fresh tissue in agarose then slicing with a vibratome - I just wondered how...

01 August 2019 5,731 3 View