Hello, I have been reviewing several articles on DNA methylation quantification for aging studies. I don't want to do a complete genome DNA methylation quantification, since it is quite costly, but how can I do a site-specific DNA methylation quantification? Most PCR-centered methods offer only methylated/not-methylated assessment which I don't think is good enough for age quantification. The bisulfite transformation seems to be the golden standard, but by the end of it, it only quantifies methylated/not-methylated analysis. Am I missing something?
Thanks a million in advance for your help
Hi Amal,
have you check the double standard DNA damage biomarker?
if so, have you check cell cycle arrest?
finally, have you check senescent biomarkers such as p53, p16, β-gal and telomere length?
I think answering these question will lead you to a better answer regrading the aging
thanks
Thanks Massar, of course there are several markers for cellular aging, such as senescence markers as well as telomere markers in addition to inflammation and fibrosis... but the golden standard for aging assessment is DNA methylation. There are several aging clocks today that can correctly assess your biological age, versus the chronological one based on DNA methylation, and predict your life span within 2-3 years margin...
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