Mosquito homogenization medium?

Sandra Fisher-Grainger @Sandra-Fisher-Grainger

24 May 2019 1 3K Report

Is anyone familiar with BA-1 diluent as referred to by Lanciotti and others for mosquito homogenization? I'm looking for a protocol to make it. Thanks.

Idalba Serrato

Hi Sandra,

I found this: with 2 ml of BA-1 diluent (1× medium 199 with Hanks' balanced salt solution, 0.05 M Tris buffer [pH 7.6], 1% bovine serum albumin, 0.35 g of sodium bicarbonate per liter, 100 μg of streptomycin per liter, 1 μg of amphotericin B [Fungizone] per ml). https://jcm.asm.org/content/38/11/4066.long

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