Idalba Serrato

4 Questions 13 Answers 0 Followers

Questions related from Idalba Serrato

What is the correct use, or the proper buffer and concentration of MNase to degrade folded RNA?

Good morning, I am trying to degrade an RNA naturally resistant to degradation by RNases, I wanted to use MNase but it only degraded very little. My sample is in liquid medium, in 95uL of RPMI and...

24 July 2023 7,607 2 View

How I can make cDNA from RNA samples with complex structure like stem-loop and dumbbell elements?

I am trying to denature RNA samples made up of stem-loop structures and dumbbell elements, it has been a really difficult task, I already did RT-qPCR in two steps and I could not even then. Does...

12 January 2022 978 3 View

Do you know of some simple and inexpensive dyes that can be used for LAMP?

We are trying to re-standardize the test with what we can find in the country because imports are complicated and expensive due to the pandemic.

21 July 2020 5,440 0 View

I have had a problema becuse the Plasmodium doing melanized after to 7 days, What can I to do?

I need infect the salivary glands of Anopheles albimanus with Plasmodium but I have had a problema becuse the parasites doing melanized after to 7 days, What can I to do?

04 May 2016 8,679 0 View