Which methodology is most recommended to perform a gene knockdown in human monocytes- derived dendritic cells: morpholino or siRNAs?
There is more data on siRNA- they have been discovered earlier. They degrade faster and are easier to transfuse as their structure is more "natural", therefore easier to pull through cell membrane.
Morpholino are harder to transfuse effectively due to low charge of backbone, and lack of affinity to any membrane proteins, but they are more stable to enzymatic degradation and environmental factors.
In my opinion a continious repression should be preferred, e.g. via using an shRNA-vector.
If you mean morpholino vs siRNA, I would prefer siRNAs, because this method/tool is more widely spread and accepted.
It is true - if you just look for knockdown in DCs, there seems to be more data on diRNA including these two:
Article An efficient siRNA-mediated gene silencing in primary human ...
http://www.jimmunol.org/content/178/9/5454
one using lipd-based transfection one using electroporation.
(even though I dont like the WB in the latter publication).
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