Hi everyone,
I do not have much experience of monoclonal antibody production. We have just ordered an external service to produce for us. The problem is that our immunogen is only 4 aa peptides, resulting in poor binding of fusioned cells to the targeted protein. However, there is binding between these clones with a larger peptide of 15 aa containing the 4 aa of interests.
Should we give it a shot and continue the project, or terminate it?
Thank you very much for helping.
Best regards,
Ly
Apparently, you have an immune response. So, I'd go for it. Have you tested if your immunogen competes?
In ELISA, just add the immunogen and target protein before you add the antibody. Ideally, mix immunogen and protein before you add it to the plate, esp. if you have a sandwich ELISA with a coated antibody.
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