Do u have any tips for blotting of integrin b1?

I blocked with skimmed milk, did the 1ry Abs incubation overnight at 4C. I have used two Abs (two membrane) for detection of integrin b1 using western blotting. Washed 5 times with TBST, each is...

30 November 2020 4,236 3 View

How can I extract high quality bacteria genomic DNA from sulfate reducing bacteria (SRB)?

All step from culture bacteria, which is the suitable media, and the age of bacteria.

22 September 2019 244 1 View

Hi..,can anyone help me to count blastomeres in an embryo image on day 3 (that's with 8 cell) ??????

here is attached images that i want to count cells on >>>if there is a Matlab code do this????

15 March 2018 3,055 6 View

What is the meaning that cancerstemcell ratio do not change by treatment with herbal extract?

what is the meaning that cancerstemcell ratio do not change by treatment with herbal EXTRACT?

01 December 2017 7,994 2 View

Why number of cancerstemcells increase from .5% to16.5% in the presence of herbal extract (that expected to decrease cancerstemcells)

Why number of cancerstemcells increase from .5% to16.5% in the presence of herbal extract ( that expected to decrease number of cancerstemcells)

02 August 2017 1,857 3 View

What is the difference between T47D and SK-BR3 breast cancer cell lines?

T47D and SK-BR3 are both breast cancer cell lines, but what are the differences between them? and also what are the differences regarding the cells shap under microscope?

28 February 2017 4,211 2 View

How to predict if specific protein can bind to another specific protein?

31 December 2015 3,398 1 View

How can I get a list of miRNAs on a chromosomal band?

12 October 2015 5,094 3 View

How can I get a list of miRNAs on a chromosomal band?

09 October 2015 3,014 4 View

How can I get the chromosomal location of a list of genes?

I am looking for a bioinformatic website or tool to get the locations of a list of genes but in one shot instead of doing it gene by gene. Is there a way to do this? Also, if you have R script to...

04 May 2015 5,183 17 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View