Good day everyone !

I'm having trouble using MitoTracker CMXRos on Arabidopsis thaliana seedlings roots (7 days old).

I'm using the dye at a concentration of 250 nM in liquid MS medium and with 30 min of incubation (with shaking).

But I never observe any signal using a confocal microscope with an "RFP" excitation (558 nm) setting.

Comparing with the literature, I never saw any particular step that would be required to stain the roots. Has anyone experimented the same issue? What protocol do you usually do to strain roots?

Thank you for your help.