I'm observing dense bodies in the mitochondria of my knockout mice- under TEM no other major defects by phenotype have been observed (these mice do exhibit a great deal of inflammation at the time of sacrifice).
I've put an image up under a link to a google document: https://docs.google.com/document/d/1JsPdqZpIgRuS2PLgOOXmhpOgNtYPcdtZm4D4JeMWHd0/edit?usp=sharing
Any ideas on what I'm seeing? Lysosomal activity?
I would have thought that those were Osmium stains due to some technical problem if they were not all located in mitochondria. So the Osmium stain is not a valid idea. They are not lysosomes either. I am afraid you will have to repeat your experiment to make sure it is not a technical glitch.
Thanks for the response! I don't think it's a glitch since it's been repeated over more than a dozen mice now (half knockout, half control), in different batches of staining and sample inclusion, and it pretty consistently presents in the same manner exclusive to the knockout mice seen in the linked image.
We have seen very similar structures in our mitochondria in mouse tissue that undergo our experimental model. The literature does have a few references to them but it seems no one really has much of an idea as to what they are. Our best guess is that it relates to some sort of dysfunction in the mitochondria as we don't see it in our control mice, similar to your situation.
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