Mitochondria witout crista (distrupted crista) or technical issue?
I'm wondering what I see in my TEM images about the mitochodnria. I know there is some diseases where the cells can contain mitochondria with partial crista remains or without crista at all, is it also in my case?
I'm doing a research about a human disease, it's PBMCs from blood.
What is your opinion?
Good day!
Mitochondria can lose cristae as a result of swelling or hypertrophy, such as necrosis or membrane permeability to ions (when Na / K ATPase or Ca2+ ATPase stops working).
Hello,
Some crista are there but barely visible. there is not enough contrast in the mitochondria.
Hello Linoy! Mitochondria (Mt) are morphologically complex structures, often elongated and branching, therefore, they are usually cut in various planes in one ultrathin section. One profile of the Mt, seen in the upper part of the cytoplasm in this view, is absolutely normal, having transversally oriented cristae in the dense matrix. But if the Mt is long enough, it may contain parts with reduced or even absent cristae depending on the functional status of this structure or the cell itself. It is usually necessary to assess many profiles of cut Mt in a sample and evaluate the proportion of normal-looking and empty-looking Mt in order to draw some conclusions about their function. But I do not see any signs of technical issue in this electron micrograph. Dimitri
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