What is the reason for arising of a negative peak during a sample run in an HPLC system?

What could be the reason behind a negative peak mostly arising during the initial stage of sample run (RT 1-10).

15 February 2021 4,231 4 View

How do I scale up a column for preparative isolation/purification from analytical conditions in HPLC?

What protocol should I follow to determine optimum separation conditions using an analytical column in an HPLC system?

03 February 2021 7,288 3 View

How to calculate the polysaccharide content of a curcuma species through HPLC method?

what all standards are needed to quantify and analyze the composition with a proper method fie for the mobile phase to run the sample ?

31 January 2021 7,704 4 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

How can I check the chemical composition of an essential oil using HPLC analysis?

Injected an oil having a concentration of 832mg/ml and found a flat top peak and was not able to quantify. How to resolve this problem?

20 January 2021 8,461 3 View

How to simulate desiccation in molecular dynamics?

I'm trying to study the effects of desiccation on some proteins, could someone tell what solvent or what simulation conditions I should set up to simulate a desiccation environment on GROMACS.

05 January 2021 7,033 3 View

What is diffrence between coupling buffer and immobilization buffer in SPR experiment?

i am performing SPR experiment and i am referring some journal and websites they stated regarding coupling buffer and immobilization buffer so ihave confusion about these buffer.

06 December 2020 470 3 View

What is the scope of doing PhD in Virology in the UK?

With the great interest in Virology in the world due to the COVID-19 pandemic and its related vaccine development is doing a Phd in Virology from a good institute in UK the right thing to do?

26 November 2020 3,681 3 View

Is it a feasible way to model CO2 emissions Mathematically?

I am working on the Environmental and Social indicators. So the easiest way is to get the solution is to go for any LCA tool;But my doubt is ,is it possible to mathematically model the Emissions...

17 November 2020 8,492 3 View

How to fix this gromacs error?

Residue 6 named GLU of a molecule in the input file was mapped to an entry in the topology database, but the atom CG used in that entry is not found in the input file. Perhaps your atom and/or...

16 November 2020 1,110 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View