MiRNAs in idiopathic non obstructive azoospermia ?

19 March 2025 0 8K Report

searching on effects of differtially expressed miRNAS in idiopathic non obstructive azoospermia leading to major caue of male infertility.

Badges
Science topic

More Huma Salahuddin's questions See All

Being healthcare Provider , im student of EMBA in healthcare management at lumhs jamshoro, need help in choosing topic for research article?

Being healthcare Provider, i need help for research article related to health problems in content to management ?

03 November 2023 7,117 6 View

How we can calculate the Formation energy of a single perovskite material such as MgFeH3 in KJ/mol.H2? I have calculated in eV/atom. I need KJ/mol.H2?

How we can calculate the Formation energy of a single perovskite material such as MgFeH3 in KJ/mol.H2? I have calculated in eV/atom. Please guide me on how I can convert -18.1 eV/atom to KJ/mol.H2

30 September 2023 7,838 1 View

How can i combine RNAseq gene expression data from fresh frozen and FFPE samples?

I want to compare baseline vs relapsed samples in an RNAseq cohort with FFPE (14) and some fresh frozen (7) samples. From clustering, it seems that FFPE and FF (fresh frozen) samples are forming...

20 November 2022 7,840 1 View

How can we calculate Spectroscopic limited maximum efficiency (SLME) by using CASTEP code?

04 August 2022 7,967 0 View

How can i measure the resistivity of the mgo nanowires deposited on the silicon substrate?

how can i measure the resistivity of the mgo nanowires deposited on the silicon substrate?

08 June 2022 6,527 2 View

How we can depsoit electrode on susbtrate ?

i wnt to deposit Au or Al electrode on my silsicon substrate deposited with nanowires can any one help me how i can do this by myself the companies iam approaching are very expensive ..

11 May 2022 952 8 View

XRD of MgO nanowires deposited on silicon susbstrate?

i need to know the exact step size and scan speed for the MgO nanowires xrd deposited on the silicon susbtrate whenever i do xrd it just shows silicon peak and MgO peaks gets vanished at the end...

28 March 2022 3,751 4 View

What could be the most common genetic reasons of segregation in the parental genotypes?

Among wheat or other self pollinated cereal crops

13 February 2022 1,159 8 View

How to interpret if the path from X to M is negative and M to Y is also negative but the indirect effect come out positive? how can i interpret it?

I have a model in which there is a negative relationship between IV and mediator and mediator to the outcome. But the indirect effect in case of mediation analysis comes out as positive. how it...

12 January 2022 5,435 2 View

How can i Etching Nanowires from the silicone substrate?

i want to etch the MgO nanowires from the silicon substrate deposited with cvd i need to transfer the naonwires to the glass subastrate to deposit electrode anyone can help please ?

14 December 2021 7,711 7 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

Does anybody face problems with total RNA isolated after miRNA mimic and inhibitor transfection?

Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...

13 May 2024 4,935 7 View

Why do many women talk of lovemaking to rather than sex?

It is difficult for most males to comprehend that females are not aroused by seeing male genitalia. Some males never come to comprehend this. (Alfred Kinsey)

11 May 2024 5,981 0 View