They are not pure lines yet and still have some hetergenous alleles to segregate.
A pure line has been defined as the progeny of a single self-fertilized homozygous individual. A complete pure line is supposed to be homozygous at all loci. In practice, we deal with partial genotype and partial phenotype. A line or parent may be pure (carrying similar alleles) at one, two, three, or more number of loci (characters), while retaining considerable "heterozygosity" for several QTLs (quantitative characters). Therefore, segregation is often observed in a seeming pure line parent in self-pollinated crops like wheat. However, if we assume 100% homozygosity (which is almost impossible), germinal mutation can cause heterozygosity for a locus (trait), resulting in segregation in the offspring. Without being heterozygous at one or more loci, a parent cannot segregate at gametogenesis.
Dear Dr Huma Tariq Dr . See the following useful RG link: Article Genetic Properties Responsible for the Transgressive Segrega...
Kindly see also the following very good link: Article Segregation distortion: Utilizing simulated genotyping data ...
Also check please the following good RG link: Article Segregation Analysis Suggests That a Genetic Reason May Cont...
The following RG link is also very useful: Article Genetic map construction and functional characterization of ...
the level of the ploidy as well as the heterozygosity. In some cases, this has to do with the LD.
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