Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

What are the effective tech-collaborative-tasks for developing reading skill?

Collaboration is essential for ensuring learning for all learners in one classroom. Blackboard collaboration tools like blogs, wikis can be considered the best platform for increasing interaction...

17 February 2020 2,991 3 View

How to synchronise two signal with different sampling frequency?

I have two bio signal. One sampled at 1KHz and another is 2 KHz. How can I synchronize these two signals. I want to use Matlab for this. Thank you.

26 August 2019 5,692 5 View

Why my PCR band is so weak?

I do PCR reaction for my gene (it's 413bp). my pcr bands are so weak. I examined different template concentration (template is FMDV cDNA, 4900 nt),increase Mgcl2 concentration, and reduce primeres...

04 December 2018 4,772 15 View

Hello, Is it possible to generate a mice model having combined features of Agt-overexpression, overectomized and breast tumor development?

To mimic human model of breast cancer after post menopause

02 May 2017 5,891 3 View

Does ABAQUS software give me sound absorption coefficient of the materials directly without adding any any code itself?

13 February 2016 8,286 10 View

In all optical autocorrelation for femto-second pulse measurement: Why is a nonlinear SHG crystal situated in front of the detector?

01 January 2016 7,340 3 View

What are the advantages and disadvantages of Stable versus unstable resonator?

05 March 2015 8,961 4 View

Is there any relation between coherence and polarization as two distinct properties of light?

To my knowledge, there is no solid correlation between temporal or spatial coherence and the polarization of light (linear, circular or elliptical) because the coherence arises from the nature of...

04 January 2015 3,902 12 View

Does anyone know how to make Fluorapatite with Phosphoric Acid?

I am Interested in making Fluorapatite.

24 August 2014 3,948 8 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

3T3-L1 cells, is this a bacterial contamination or a cross contamination with different cell line? or something else?

Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...

13 February 2021 4,040 3 View

What can be use to mop up heavy metals from vegetables or soils in quarry mining site?

It has been established that quarry mining site polluted and contaminated it's environment with heavy metals, these affects the crops and vegetables planted in the area. The possible solutions to...

12 February 2021 1,155 5 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

Resume Book UEP2013 Asian Edition, dis somebodey has it?

I am looking for a copy of the resume book of UEP2013 Asian Edition (Urban Environmental Pollution 2013 Asian Edition) held in Beijing. If somebody has it, please I would like to obtain an...

11 February 2021 2,247 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View