The tissue we are using is the cerebellum of black mice blc57, we are doing histopathology and oxidative stress assay, Should we use half the number of mice for each test or could we divide the cerebellum and do both tests for the whole number?
I used to primary cell culture for mice and humans. In general, a trend shows up about 5 samples each if there is any change. You will need more than 10 (10 controls and 10 of your condition). That will determine how many mice you need. BE sure there is a significant variation among each mouse. The following papers are not the brain but may be useful:
Article Multiplex stress resistance in cells from long-lived dwarf mice
Article Fibroblast cell lines from young adult mice of long-lived mu...
Nedaa Kiwan , You need to take 5 separate samples from each group of mice. I agree with Shin Murakami
