Hello,
I'm working on drug delivery involving microfluidic double emulsions production and I'm looking for a way to monitor the production of the droplet in live to be able to get the frequency, and the inner/outer diameter of the emulsion produced in real time, in order to change the flow and reach the aimed dimensions.
I used a system involving lasers, PMT and fluorophores before but I was wondering if it was possible to develop an homemade softwre/system on the brightfield images using an high speed camera couplet with ImageJ + Matlab or R.
Has anyone done something similar before, and can help me or give me advices to develop it ? Do you think the real time aspect can work with the high speed camera in brightfield ? Is Matlab more suitable for this (I'm pretty familiar with R/Shiny but not with Matlab) or is there any other software that can be used for this application ?
Many thanks for your help,
Best,
Romain
Dear Romain Guyon,
It is possible to use MATLAB to perform this type of monitoring using MATLAB Image Processing Toolbox™ and Signal Processing Toolbox™. Before you decide whether to use MATLAB or not, you should check the compatibility of the camera with MATLAB, the number of frames per seconds, the size of the image, and other issues that can be further found in the following articles:
https://www.mathworks.com/company/newsletters/articles/analyzing-high-speed-video-images-to-quantify-the-performance-of-innovative-drug-delivery-technology.html
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Dear Marwan,
Many thanks for your answer, it's really useful. The articles are really interesting, and I guess I could try to do a similar approach as the nature's paper, maybe simplified as microfluidics emulsions should be circular and I just need the inner and outer sizes of the droplet.
I will check compatibility between the camera and Matlab and see if there is tutorials/other articles to develop the size monitoring process, as I need to learn Matlab code.
Thanks again !
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