What can i do to extract antimicrobial compound from bacterial medium supernatant if do not know the nature of antimicrobial compound?

I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...

21 September 2020 6,869 3 View

How to identify fake research and predatory journal?

Fake researches and predatory journals

14 September 2020 4,530 7 View

For Scanning electron microscopy based trichome analysis of the leaf samples fresh samples can be preserved?

For SEM-based analysis of trichomes (soybean plant) can we preserve the samples collected for some period of time? or after following standard fixation procedures it can be preserved under low...

01 June 2020 5,665 1 View

New problem Statement on Solar Energy?

Dear All, I want to know the new research topic on Solar Energy for my PhD. work. Please let me know.

13 January 2020 4,388 1 View

What is the appropriate way / index of expressing dissimilarity of elements in a large sample collection?

Ex. I have taken a sample of x papers. Total number of contributing authors in these x papers are N authors. However, some authors have contributed in more than one papers in the sample. Say the...

03 July 2019 9,941 1 View

What is the lowest concentration you should take for determination of solubility of compounds by LC-MS method?

I have to determine solubility of LC-MS of completely new compound, however, the sample provided is very low. So, I would like to know what is the safe concentration to start.

07 February 2019 6,945 3 View

How to determine the connectivity of the pores through BET Isotherm analysis? How do we know the pores of the material are interconnected or isolated?

I have synthesized a series of porous organic polymers by tuning the linker length and observed a trend of variation of surface area (ultramicroporous, high surface area to mesoporous, low surface...

25 November 2018 3,962 2 View

Resuspend salivary proteins in 1X Coupling Buffer???

I have to enrich glycoproteins from salivary samples. I am using hydrazide resin. But it's very difficult to resuspend salivary proteins in 1X coupling buffer. Is there any solution for it?

07 September 2017 3,897 2 View

How to obtain ethical clearance for a multi-center study?

We are planning to conduct a questionnaire-based study among dental students of different dental colleges of Nepal (within a country). Should we obtain ethical clearance from each institution's...

03 October 2016 7,434 5 View

How to check titre of egg yolk antibody against bacteria?

How can I determine the titre of my egg yolk antibody against bacteria..or can anyone plz tell me how to check antibody titre against bacteria.thank you?

08 July 2016 3,224 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

How commonly do you encounter Rummination Syndrome in your GI practice?

Do diaphragmatic breathing and speech therapy actually help? How often did you require multidisciplinary team approach?

17 January 2021 5,998 0 View

Does use of hand sanitizer negatively impact the skin microbiome?

Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...

16 January 2021 6,923 11 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Fish gut microbiota extraction and identification protocol?

Hi, I need a protocol to extract and identify microbiota from fish gut. Any suggestions? Thank you. (P.s. Something low tech as I am technologically limited, guess no funds and big high tech...

01 January 2021 808 1 View

Are two text files obtained separately from qiime 2 can be used together to make diversity graphs?

I have two sets of 16s microbiome sequencing data. The animal model, facility, diet vendor are the same in both sets. The only change is in the treatment. For analysis, can I use both the...

29 December 2020 6,976 3 View

Does anyone know the neutrophil marker for qPCR in human gut (duodenum, ileum, colon) biopsies?

Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...

25 November 2020 2,680 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View