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Questions related from Chankit Giri
Elution buffer composition is- 50mM NaH2Po4, 300mM NaCl, 250mM immidazole at pH 8. PI of my protein is 6.7. I purified My protein using Ni-NTA resin and run sds page and till the completion of gel...
24 February 2023 4,658 12 View
I am working on the transcription factor. I am trying to get protein and for that, I did molecular cloning of gene into e.coli. But I am facing a problem that protein is going into the pellet. I...
27 July 2022 8,648 11 View
I am trying to digest pET28a by Bam HI and Xho I. I am using 1.5 ug DNA and 1ul of enzymes (which ia 20 unit per ul) and incubated at 37*c for 6 hours and in second reaction, incubated for 2...
29 August 2021 6,224 5 View
I have tried many things like, took medium supernatant and added with hexane, ethyl acetate, chloroform but none of these are able to extract antimicrobial compound from supernatant.. I also did...
13 October 2020 7,635 0 View
I am having an unknown bacterial hit so far, which is inhibiting the growth of multidrug-resistant bacteria. I want to characterize that antimicrobial compound.what should I do to characterize...
22 September 2020 6,983 3 View
How to extract secondary metabolite/ antimicrobial compound from bacterial medium supernatant if do not know the nature of metabolite. I used ethanol, ethyl acetate, chloroform, hexane for...
16 September 2020 5,841 4 View
Any one can suggest some related ideas on the basis of gut microbiome, as i am having interest in it.. i am looking for one of my objective in microbiome study for my PhD thesis..
20 February 2020 2,227 4 View
How can I determine the titre of my egg yolk antibody against bacteria..or can anyone plz tell me how to check antibody titre against bacteria.thank you?
09 July 2016 3,327 3 View
how can i purify the chicken antibody IgY from an egg..i want good yeild..and i want to go in bulk purification.i am using PEG6000 now a days,but yeild is not good..i want to purify view other...
06 June 2016 5,141 3 View
which probiotic is best for a shrimp to protect this by bacterial or viral pathogenic microorganism..and what the mechanism behind that.i have the antibody of a bacterial pathogen of shrimp..which...
01 June 2016 8,034 4 View
