Methods for the separation of polyphenols, and anthocyanins from legume seeds (with seed coat).

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Robin Joshi

For anthocyanin extraction use 0.1%CH3COOH in methanol because acidic condition is stable for anthocyanins. Then use XAD and purify on PrepHPLC.

For other phenolic compounds you have to use 70%methanol and purify on C-18.

Gud luck

Abhinay Bacha

Thanks for the info sir..

Simona Conea

Extract anthocyanins with C2H5OH 95c : HCl 1,5 N (85: 15, v/v) then keep the solution for 1 day to 4 C.

thanks for the info, @ Simona and Luisa