Mari Johnson

6 Questions 7 Answers 0 Followers

Questions related from Mari Johnson

Methods for intergrating whole blood and PBMC RNA-Seq data?

Hi - I'm currently working with two RNA-Seq studies; one has RNA extracted from whole blood, the other PBMCs. Eventually we want to combine these data and perform some cell-specific deconvolution...

17 March 2023 9,736 3 View

How can I merge two PED/MAP file sets from different chromosome builds for GWAS study in PLINK?

Hi, I have two data sets from the illumina omniexpress snp array platform. The first data set was mapped using the GRCh37 build and the second one was more recently read using the GRCh38 build....

15 June 2020 6,593 1 View

Can you fix cells with Dihydrorhodamine for a respiratory burst assay?

Hi, I'm running an assay measuring phagocyotosis and ROS production in PBMC's. I'm using Dihydrorhodamine 123 (Thermo) to measure ROS. I then stain and fix with PFA afterwards. I've so far read...

15 October 2019 6,240 5 View

How to make a plasmid stock from old plate?

So unfortunately I have low amounts of DNA used for my transformation. I still have the plates so was wondering if it's best to just pick a colony from that plate, grow an O/N. make a fresh plate...

07 January 2018 4,623 4 View

Can you leave a bacterial glycerol stock in the fridge?

Apologies for the probably stupid question but I'm new to the field and forgot to put my glycerol stocks in the freezer, will they be ok over the weekend? Thanks for any help!

23 July 2016 5,563 4 View

Very low concentration DNA after restriction digest?

We used a small amount of our digest (5ul out of 100) and ran on the gel to confirm the digests success, when the rest of the digest sample was quantified with nano drop however, no DNA could be...

01 July 2016 5,158 13 View