I am interested in a method to measure Thioredoxin activity and/or Thioredoxin reductase activity by spectrophotometry without the use of insulin in vegetable tissues
Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...
07 July 2024 1,685 0 View
Which membrane material is the best for capturing and visualizing phosphorylated proteins in a western blot? Or is there no difference?
05 June 2024 9,502 2 View
Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...
03 June 2024 6,795 1 View
Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...
08 May 2024 5,665 0 View
I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...
06 May 2024 8,974 5 View
Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...
11 April 2024 4,457 1 View
There are significant challenges that the blood bank industry in Kenya must overcome in order to be able to maintain a balanced equilibrium between the demand for blood and the supply of blood....
10 April 2024 974 2 View
Based on the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan, et,al. (2023)
09 April 2024 1,796 1 View
I wonder if there has been a progress in the way that conservators treat the metallic threads, especially the ones that are associated on liturgic textiles.
17 March 2024 4,773 0 View
Esta pregunta se refiere ha como podemos saber, si después de una intervención, ya sea en grupo o individualmente, a la hora de tratar con adolescentes problemáticos, nuestra intervención a sido...
11 March 2024 7,801 2 View
I tried to isolate pr-FMN from UbiD like enzyme and verify it via UPLC and Mass spectrometry. The results obtained from MS shows detection of right mass but UPLC spectrum tell another story. How...
17 March 2024 4,645 3 View
The anti-aging gene Sirtuin 1 is associated with the reversal of cell senescence and apoptosis with relevance to many organs and tissues. Sirtuin 1 repression is linked to the global chronic...
27 February 2024 5,762 0 View
I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...
18 February 2024 3,338 0 View
I am trying to express HIV1 antisense protein in E.Coli. The protein is hydrophobic and also has two N-terminal cysteine triplets. I am planning to add a pelb signal peptide for periplasmic...
31 January 2024 1,862 1 View
In thiol-disulfide exchange reaction, pyridine-2-thione is expected to be released as the reaction proceeds giving yellow color to the medium, and the Absorbance should increase and then become...
15 January 2024 689 0 View
I am trying to purify a protein using thioredoxin tag. The clone I made is in pET32b vector having N-terminal thioredxin tag and C-terminal His-tag. I already checked the plasmid using...
06 November 2023 9,339 3 View
hello I wane to interpolate temperature in an alpine region (valle d aosta ) , i want to know how i can obtain better results ?i have data of 87 meteorological stations that distributed in an...
03 October 2023 6,309 9 View
I want to prepare a calibration curve from BTEX standard for volatile organic compounds analysis by GC-FID and I have a problem related to its preparation in carbon disulfide. The standard comes...
16 August 2023 9,612 0 View
I would like to performe the GRIESS reagent kit from biotium. The nitrate reductase enzyme (Sigma catalog no. N7265) has an activity ≥300 U/g. By protocol I am to use it at a final concentration...
21 July 2023 1,182 3 View
Glutathione Reductase (GR): Glutathione Reductase is an enzyme that plays a crucial role in the glutathione cycle and the cellular antioxidant defense system. It is responsible for converting...
20 July 2023 6,781 0 View