How to extract peak coordinates from a given cluster with MATLAB as in the SPM results viewer?

Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...

26 January 2021 7,672 2 View

My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

Is there any index number for first author in journal article like h index?

In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?

15 December 2020 8,231 14 View

How to identify original journal website?

There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...

18 November 2020 7,650 6 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Appropriate stats test for multiple DV, multiple treatments, paired across days?

Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...

03 July 2020 4,487 7 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Is there any correlation to relate temperature and shear strength of Aluminum alloy AA2024-T4?

As shear strength of AA2024-T4 alloy is inversely proportional to the temperature. is there any equation to predict shear strength of AA2024-T4 with respect to temperature?

12 May 2020 8,804 2 View

What should be the ideal Thiolated-Oligo:GNP ratio for effective conjugation?

I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.

08 December 2020 7,975 2 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

How important is Bempedoic acid in reducing LDL-C in patients who are not responding well to maximal doses of statins?

Bempedoic acid inhibits ATP citrate lyase (ACL), an enzyme which converts Citrate to Acetyl CoA . After this step, the pathway is same i.e. Acetyl CoA to HMG CoA and mevalonate (targeted by...

22 January 2020 9,687 3 View

Impact of change in temperature on adsorption of CS2, in the presence of water vapor on activated carbon at constant partial pressure of CS2 and H20?

For pure component adsorption of CS2, the adsorbed quantity increases with decrease in temperature using the Dubinin-Radushkevic (D-R) equation. Similarly for pure component adsorption of H2O...

06 July 2019 5,120 1 View

Complete Protocol for Glutamine synthetase, Glutamate dehydrogenase and nitrate reductase enzymes?

My research is to assess nitrogen use efficiency in different wheat genotypes by determining the activity of ammonium assimilation enzymes which are mentioned above. But after a lot of struggle I...

23 May 2019 7,122 6 View

Complete Protocol for Glutamine synthetase, Glutamate dehydrogenase and nitrate reductase enzymes?

My research is to assess nitrogen use efficiency in different wheat genotypes by determining the activity of ammonium assimilation enzymes which are mentioned above. But after a lot of struggle I...

04 May 2019 9,978 6 View

What is the difference between INT and MTT?

For cell viability detection, I have tried using INT so far with the protocol of 0.5 mg/l in PBS. after adding 1 ml of prepared INT solution to my sample containing adhered E.coli bacteria I...

02 March 2019 8,916 1 View

Are there any effects of thioredoxine tag (Trx-tag) in pET32a on immune response and efficacy of vaccine in animal?

I am studying about recombinant protein to produce vaccine, and I use pET32a as vector for protein expression. I wanna ask are there any effect of the thioredoxine tag (Trx-Tag) in the immune...

02 March 2019 1,343 3 View

How can I accurately measure the Free Thiol content of HSA?

I have been attempting to calculate the mol/mol ratio of free thiol to protein for HSA, but my results have not yielded a value close to that of the accepted value. We have seen various values...

31 December 2018 7,286 3 View