Hello, looking for TrpV1 knockout extracts from rat or human cells anyone have any?

Hello, I am testing some TrpV1 antibodies and would like to use KO extracts. I can find some whole mice but they are a bit steep for just some westerns. Does anyone have some KO extracts from...

09 October 2019 9,798 1 View

Can anyone recommend a good ELISA for COL2A1 (human)?

I have a small amount of money and time left on my project and would like to do an ELISA. I'm struggling to find one with any reviews and none of my colleagues can recommend one.

03 December 2018 2,508 2 View

Why does the mean droplet size of an emulsion changes with increasing the concentration of solid particles?

I am evaluating the effect of nanoparticle on emulsions. It was observed that when the increasing the concentration of the nanoparticles changes the mean droplet size (morphology). What is the...

24 July 2018 5,502 13 View

Neural network outputting average values of all outputs - Am I doing anything wrong?

14 January 2016 6,803 8 View

I am looking to compile a citation reference list for RNA-Seq; has anyone started one? Would anyone like to collaborate?

Hello, I would like to compile a citation reference list covering human RNA-Seq needs for researchers. I am wondering if anyone has already compiled a "guide line" list for the sequencing depth...

10 January 2016 1,370 2 View

How do I find degenerate PCR primers?

31 August 2015 3,824 3 View

What are the best non-metabolism based proliferation assays for cancer cells?

I have previously used CFSE for measuring the proliferation of lymphocytes. I now want to look at the proliferation of solid malignancy cancer cell lines, but I have read that CFSE may not be...

04 June 2014 3,545 5 View

Would another 50-ish students help?

We can probably add this to our preservice course. [email protected]

01 January 1970 364 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View