Salam (Greetings),
I'm working on Metformin and wanna test its effect on some cell lines when combined with other drugs, which are mainly soluble in DMSO.
I've looked up metformin solubility and found it soluble in both water (up to 2 M) and, to less extent, in DMSO (up to 100 mM).
My question is: which one should I use to prepare it?
- Both stock and serial dilutions with DMSO, while maintaining its concentration
Hello Mariam Ahmed Galal
Water is the most commonly used and most desirable solvent without any toxic effects on cells. I suggest if Metformin is readily soluble in water, you should use water to prepare both stock and serial dilutions. Drugs which are insoluble in water may be dissolved in organic solvents like DMSO as the next possible option and serially diluted in water or culture media so that the final concentration of DMSO in culture is less than 0.1%.
Best.
The choice of solvent for metformin will depend on several factors, including the concentration of metformin needed for your experiments, the solubility of the other drugs you are combining with metformin, and the potential toxicity of the solvent to your cell lines.
Metformin is soluble in both water and DMSO, but it is more soluble in DMSO, which can be beneficial when preparing high concentrations of the drug. However, DMSO is a potent solvent that can have toxic effects on cells, especially at high concentrations. Therefore, it is generally recommended to keep the DMSO concentration in the culture media below 0.5%.
If you need to prepare high concentrations of metformin, it may be more convenient to prepare a stock solution in DMSO and then make serial dilutions in water. This will help to minimize the concentration of DMSO in your final treatment solutions.
On the other hand, if you only need low-to-moderate concentrations of metformin, it may be more appropriate to prepare a stock solution in water and make serial dilutions in water as well. This will help to minimize the potential toxic effects of DMSO on your cells.
It is important to consider the solubility of the other drugs you are combining with metformin, as some drugs may not be soluble in water or DMSO. In such cases, you may need to use a different solvent, or adjust the conditions to achieve sufficient solubility.
In summary, the choice of solvent for metformin will depend on several factors, including the concentration of metformin needed for your experiments, the solubility of other drugs, and the potential toxicity of the solvent. You may need to experiment with different conditions to determine the best approach for your specific needs.
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