Hello,

I just diffracted my new crystals and got the structure from Phaser and Autobuild which yields the LLG score of ~3900 and the TFZ score of ~50.

When scanning the structure and the electron density on Coot, I found out that the electron densities on side chains of several residues located at the outer side of the protein lack to fully describe the structure. The electron density is only capable of following the alpha carbons at those residues on the outer side.

1. Due to the reason above, I am trying to merge the sca files from HKL2000 or the mtz files, which are basically from the sca files, from other crystals which are supposed to have similar cell parameters.

2. In addition, I was trying to remove some bad frames from my 360 image set which was used for the structure mentioned at first. What I tried is to remove those frames manually and tried to integrate them but they were separated before and after the deleted frames due to the lack of continuity, I guess.

Or please suggest me something to do for having better structure. I am attaching some results of what I have.