Hi
Dear All,
I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry et al., 1951) . But for measuring Total immunoglobulin whole-body extraction samples using the Lowry method, with modification by Siwicki and Anderson ( 1994 ), according to the following article:
Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis
For Total immunoglobulin When I add Folin-Ceiocalteu, the solution becomes turbid. what is the reason? What solutions would you recommend for solving this problem?
It should be noted that I performed this test three times with polyethylene glycol 6,000, 8,000 and 10,000 kDa, and in all cases the solution was turbid.
Thank you very much for your help.
Fatemeh
You can dilute the solution to 5 times and in the final readings multiply by dilution factor ..
I agree with Abhishek. Some other considerations: spin down the sample to remove particulates. Warm the sample up to room temperature.
Dear Abhishek Mathur
I before measuring protein concentration of the whole-body extraction diluted the whole body extraction samples with PBS in 1: 50 ratio.
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